Faculty
David G. DeNardo.jpg

David G. DeNardo, PhD

Associate Professor
Department of Medicine
Oncology Division
Molecular Oncology
Department of Pathology & Immunology

Research Interests

  • Immune response
  • Metastasis
  • Breast cancer
  • Pancreatic cancer

Contact

  • 314-362-9524 (office)
  • 314-362-9534 (lab)
  • 314-747-2797 (fax)
  • Room 7611 BJCIH (office)
    Room 7207 BJCIH (lab)
  • Division of Oncology
    Campus Box 8069
    Washington University Medical School
    660 South Euclid Avenue
    St. Louis, MO 63110
  • David DeNardo Lab

Peer-reviewed Manuscripts

  • Tissue-Resident Macrophages in Pancreatic Ductal Adenocarcinoma Originate From Embryonic Hematopoiesis and Promote Tumor Progression
    Zhu Y, Herndon JM, Sojka DK, Kim KW, Knolhoff BL, Zuo C, Cullinan DR, Luo J, Bearden AR, Lavine KJ, Yokoyama WM, Hawkins WG, Fields RC, Randolph GJ, DeNardo DG
    Immunity 2017 Sep 19;47(3):597
  • Tissue-resident macrophages in pancreatic ductal adenocarcinoma originate from embryonic hematopoiesis and promote tumor progression
    Zhu Y, Herndon JM, Sojka DK, Kim KW, Knolhoff BL, Zuo C, Cullinan DR, Luo J, Bearden AR, Lavine KJ, Yokoyama WM, Hawkins WG, Fields RC, Randolph GJ, DeNardo DG
    Immunity 2017 Aug 15;47(2):323-338
  • Targeting focal adhesion kinase renders pancreatic cancers responsive to checkpoint immunotherapy
    Jiang H, Hegde S, Knolhoff BL, Zhu Y, Herndon JM, Meyer MA, Nywening TM, Hawkins WG, Shapiro IM, Weaver DT, Pachter JA, Wang-Gillam A, DeNardo DG
    Nat Med 2016 Aug;22(8):851-60
  • The action of discoidin domain receptor 2 in basal tumor cells and stromal cancer-associated fibroblasts is critical for breast cancer metastasis
    Corsa CA, Brenot A, Grither WR, Van Hove S, Loza AJ, Zhang K, Ponik SM, Liu Y, DeNardo DG, Eliceiri KW, Keely PJ, Longmore GD
    Cell Rep 2016 Jun 14;15(11):2510-23
  • Stromal senescence establishes an immunosuppressive microenvironment that drives tumorigenesis
    Ruhland MK, Loza AJ, Capietto AH, Luo X, Knolhoff BL, Flanagan KC, Belt BA, Alspach E, Leahy K, Luo J, Schaffer A, Edwards JR, Longmore G, Faccio R, DeNardo DG, Stewart SA
    Nat Commun 2016 Jun 8;7:11762
  • Constitutive IRAK4 activation underlies poor prognosis and chemoresistance in pancreatic ductal adenocarcinoma
    Zhang D, Li L, Jiang H, Knolhoff BL, Lockhart AC, Wang-Gillam A, DeNardo DG, Ruzinova MB, Lim KH
    Clin Cancer Res 2017 Apr 1;23(7):1748-1759
  • Conjugation to the sigma-2 ligand SV119 overcomes uptake blockade and converts dm-Erastin into a potent pancreatic cancer therapeutic
    Ohman KA, Hashim YM, Vangveravong S, Nywening TM, Cullinan DR, Goedegebuure SP, Liu J, Van Tine BA, Tiriac H, Tuveson DA, DeNardo DG, Spitzer D, Mach RH, Hawkins WG
    Oncotarget 2016 Jun 7;7(23):33529-41
  • Targeting focal adhesion kinase renders pancreatic cancers responsive to checkpoint immunotherapy
    Jiang H, Hegde S, Knolhoff BL, Zhu Y, Herndon JM, Meyer MA, Nywening TM, Hawkins WG, Shapiro IM, Weaver DT, Pachter JA, Wang-Gillam A, DeNardo DG
    Nat Med 2016 Aug;22(8):851-60
  • The action of discoidin domain receptor 2 in basal tumor cells and stromal cancer-associated fibroblasts is critical for breast cancer metastasis
    Corsa CA, Brenot A, Grither WR, Van Hove S, Loza AJ, Zhang K, Ponik SM, Liu Y, DeNardo DG, Eliceiri KW, Keely PJ, Longmore GD
    Cell Rep 2016 Jun 14;15(11):2510-2523
  • Stromal senescence establishes an immunosuppressive microenvironment that drives tumorigenesis
    Ruhland MK, Loza AJ, Capietto AH, Luo X, Knolhoff BL, Flanagan KC, Belt BA, Alspach E, Leahy K, Luo J, Schaffer A, Edwards JR, Longmore G, Faccio R, DeNardo DG, Stewart SA
    Nat Commun 2016 Jun 8;7:11762
  • Antagonizing integrin beta3 increases immunosuppression in cancer
    Su X, Esser AK, Amend SR, Xiang J, Xu Y, Ross MH, Fox GC, Kobayashi T, Steri V, Roomp K, Fontana F, Hurchla MA, Knolhoff BL, Meyer MA, Morgan EA, Tomasson JC, Novack JS, Zou W, Faccio R, Novack DV, Robinson SD, Teitelbaum SL, DeNardo DG, Schneider JG, Weilbaecher KN
    Cancer Res 2016 Jun 15;76(12):3484-95
  • Targeting tumour-associated macrophages with CCR2 inhibition in combination with FOLFIRINOX in patients with borderline resectable and locally advanced pancreatic cancer: a single-centre, open-label, dose-finding, non-randomised, phase 1b trial
    Nywening TM, Wang-Gillam A, Sanford DE, Belt BA, Panni RZ, Cusworth BM, Toriola AT, Nieman RK, Worley LA, Yano M, Fowler KJ, Lockhart AC, Suresh R, Tan BR, Lim KH, Fields RC, Strasberg SM, Hawkins WG, DeNardo DG, Goedegebuure SP, Linehan DC
    Lancet Oncol 2016 May;17(5):651-662
  • Dickkopf-related protein 1 (Dkk1) regulates the accumulation and function of myeloid derived suppressor cells in cancer
    D'Amico L, Mahajan S, Capietto AH, Yang Z, Zamani A, Ricci B, Bumpass DB, Meyer M, Su X, Wang-Gillam A, Weilbaecher K, Stewart SA, DeNardo DG, Faccio R
    J Exp Med 2016 May 2;213(5):827-840
  • Thymic stromal lymphopoietin blocks early stages of breast carcinogenesis
    Demehri S, Cunningham TJ, Manivasagam S, Ngo KH, Moradi Tuchayi S, Reddy R, Meyers MA, DeNardo DG, Yokoyama WM
    J Clin Invest 2016 Apr 1;126(4):1458-1470
  • TH2-polarized CD4+ T cells and macrophages limit efficacy of radiotherapy
    Shiao SL, Ruffell B, DeNardo DG, Faddegon BA, Park CC, Coussens LM
    Cancer Immunol Res 2015 May;3(5):518-525
  • Paclitaxel therapy promotes breast cancer metastasis in a TLR4-dependent manner
    Volk-Draper L, Hall K, Griggs C, Rajput S, Kohio P, DeNardo D, Ran S
    Cancer Res 2014 Oct 1;74(19):5421-34
  • CSF1/CSF1R blockade reprograms tumor-infiltrating macrophages and improves response to T-cell checkpoint immunotherapy in pancreatic cancer models
    Zhu Y, Knolhoff BL, Meyer MA, Nywening TM, West BL, Luo J, Wang-Gillam A, Goedegebuure SP, Linehan DC, DeNardo DG
    Cancer Res 2014 Sep 15;74(18):5057-69
  • Tumor-induced STAT3 activation in monocytic myeloid-derived suppressor cells enhances stemness and mesenchymal properties in human pancreatic cancer
    Panni RZ, Sanford DE, Belt BA, Mitchem JB, Worley LA, Goetz BD, Mukherjee P, Wang-Gillam A, Link DC, DeNardo DG, Goedegebuure SP, Linehan DC
    Cancer Immunol Immunother 2014 May;63(5):513-28
  • Synergistic effects of concurrent blockade of PI3K and MEK pathways in pancreatic cancer preclinical models
    Zhong H, Sanchez C, Spitrzer D, Plambeck-Suess S, Gibbs J, Hawkins WG, DeNardo D, Gao F, Pufahl RA, Lockhart AC, Xu M, Linehan D, Weber J, Wang-Gillam A
    PLoS One 2013 Oct 9;8(10):e77243
  • Cathepsin C is a tissue-specific regulator of squamous carcinogenesis
    Ruffell B, Affara NI, Cottone L, Junankar S, Johansson M, DeNardo DG, Korets L, Reinheckel T, Sloane BF, Bogyo M, Coussens LM
    Genes Dev 2013 Oct 1;27(19):2086-98
  • Targeting tumor-infiltrating macrophages to combat cancer
    Panni RZ, Linehan DC, DeNardo DG
    Immunotherapy 2013 Oct;5(10):1075-87
  • A study of zoledronic acid as neo-adjuvant, perioperative therapy in patients with resectable pancreatic ductal adenocarcinoma
    Sanford DE, Porembka MR, Panni RZ, Mitchem JB, Belt BA, Plambeck-Suess SM, Lin G, DeNardo DG, Fields RC, Hawkins WG, Strasberg SM, Lockhart AC, Wang-Gillam A, Goedegebuure SP, Linehan DC
    J Cancer Ther 2013 May;4(3):797-803
  • Inflammatory monocyte mobilization decreases patient survival in pancreatic cancer: a role for targeting the CCL2/CCR2 axis
    Sanford DE, Belt BA, Panni RZ, Mayer A, Deshpande AD, Carpenter D, Mitchem JB, Plambeck-Suess SM, Worley LA, Goetz BD, Wang-Gillam A, Eberlein TJ, DeNardo DG, Goedegebuure SP, Linehan DC
    Clin Cancer Res 2013 Jul 1;19(13):3404-15
  • Targeting tumor-infiltrating macrophages decreases tumor-initiating cells, relieves immunosuppression, and improves chemotherapeutic responses
    Mitchem JB, Brennan DJ, Knolhoff BL, Belt BA, Zhu Y, Sanford DE, Belaygorod L, Carpenter D, Collins L, Piwnica-Worms D, Hewitt S, Udupi GM, Gallagher WM, Wegner C, West BL, Wang-Gillam A, Goedegebuure P, Linehan DC, DeNardo DG
    Cancer Res 2013 Feb 1;73(3):1128-1141
  • MRI of tumor-associated macrophages with clinically applicable iron oxide nanoparticles
    Daldrup-Link HE, Golovko D, Ruffell B, DeNardo DG, Castaneda R, Ansari C, Rao J, Tikhomirov GA, Wendland MF, Corot C, Coussens LM
    Clin Cancer Res 2011 Sep 1;17(17):5695-704
  • Leukocyte complexity predicts breast cancer survival and functionally regulates response to chemotherapy
    DeNardo DG, Brennan DJ, Rexhepaj E, Ruffell B, Shiao SL, Madden SF, Gallagher WM, Wadhwani N, Keil SD, Junaid SA, Rugo HS, Hwang ES, Jirstrom K, West BL, Coussens LM
    Cancer Discov 2011 Jun 1;1:54-67
  • FcRgamma activation regulates inflammation-associated squamous carcinogenesis
    Andreu P, Johansson M, Affara NI, Pucci F, Tan T, Junankar S, Korets L, Lam J, Tawfik D, DeNardo DG, Naldini L, de Visser KE, De Palma M, Coussens LM
    Cancer Cell 2010 Feb 17;17(2):121-34
  • Macrophage expression of hypoxia-inducible factor-1 alpha suppresses T-cell function and promotes tumor progression
    Doedens AL, Stockmann C, Rubinstein MP, Liao D, Zhang N, DeNardo DG, Coussens LM, Karin M, Goldrath AW, Johnson RS
    Cancer Res 2010 Oct 1;70(19):7465-75
  • Receptor tyrosine kinase signaling favors a protumorigenic state in breast cancer cells by inhibiting the adaptive immune response
    Ursini-Siegel J, Cory S, Zuo D, Hardy WR, Rexhepaj E, Lam S, Schade B, Jirstrom K, Bjur E, Piccirillo CA, DeNardo D, Coussens LM, Brennan DJ, Gallagher WM, Park M, Pawson T, Hallett M, Muller WJ
    Cancer Res 2010 Oct 15;70(20):7776-87
  • Labeling human embryonic stem cell-derived cardiomyocytes with indocyanine green for noninvasive tracking with optical imaging: an FDA-compatible alternative to firefly luciferase
    Boddington SE, Henning TD, Jha P, Schlieve CR, Mandrussow L, DeNardo D, Bernstein HS, Ritner C, Golovko D, Lu Y, Zhao S, Daldrup-Link HE
    Cell Transplant 2010;19(1):55-65
  • Lymphocytes in cancer development: polarization towards pro-tumor immunity
    Ruffell B, DeNardo DG, Affara NI, Coussens LM
    Cytokine Growth Factor Rev 2010 Feb;21(1):3-10
  • Optical imaging of the peri-tumoral inflammatory response in breast cancer
    Sista AK, Knebel RJ, Tavri S, Johansson M, DeNardo DG, Boddington SE, Kishore SA, Ansari C, Reinhart V, Coakley FV, Coussens LM, Daldrup-Link HE
    J Transl Med 2009 Nov 11;7:94
  • CD4(+) T cells regulate pulmonary metastasis of mammary carcinomas by enhancing protumor properties of macrophages
    DeNardo DG, Barreto JB, Andreu P, Vasquez L, Tawfik D, Kolhatkar N, Coussens LM
    Cancer Cell 2009 Aug 4;16(2):91-102
  • Inflammation and breast cancer. Balancing immune response: crosstalk between adaptive and innate immune cells during breast cancer progression
    DeNardo DG, Coussens LM
    Breast Cancer Res 2007;9(4):212
  • Estrogen receptor DNA binding is not required for estrogen-induced breast cell growth
    DeNardo DG, Cuba VL, Kim H, Wu K, Lee AV, Brown PH
    Mol Cell Endocrinol 2007 Oct 15;277(1-2):13-25
  • Identification of biomarkers modulated by the rexinoid LGD1069 (bexarotene) in human breast cells using oligonucleotide arrays
    Kim HT, Kong G, DeNardo D, Li Y, Uray I, Pal S, Mohsin S, Hilsenbeck SG, Bissonnette R, Lamph WW, Johnson K, Brown PH
    Cancer Res 2006 Dec 15;66(24):12009-18
  • Stimulatory effect of genistein and apigenin on the growth of breast cancer cells correlates with their ability to activate ER alpha
    Seo HS, DeNardo DG, Jacquot Y, Laios I, Vidal DS, Zambrana CR, Leclercq G, Brown PH
    Breast Cancer Res Treat 2006 Sep;99(2):121-34
  • Global gene expression analysis of estrogen receptor transcription factor cross talk in breast cancer: identification of estrogen-induced/activator protein-1-dependent genes
    DeNardo DG, Kim HT, Hilsenbeck S, Cuba V, Tsimelzon A, Brown PH
    Mol Endocrinol 2005 Feb;19(2):362-78
  • The retinoid X receptor-selective retinoid, LGD1069, down-regulates cyclooxygenase-2 expression in human breast cells through transcription factor crosstalk: implications for molecular-based chemoprevention
    Kong G, Kim HT, Wu K, DeNardo D, Hilsenbeck SG, Xu XC, Lamph WW, Bissonnette R, Dannenberg AJ, Brown PH
    Cancer Res 2005 Apr 15;65(8):3462-9
  • Anti-HLA-DR/anti-DOTA diabody construction in a modular gene design platform: bispecific antibodies for pretargeted radioimmunotherapy
    DeNardo DG, Xiong CY, Shi XB, DeNardo GL, DeNardo SJ
    Cancer Biother Radiopharm 2001 Dec;16(6):525-35
  • Antibody phage libraries for the next generation of tumor targeting radioimmunotherapeutics
    DeNardo SJ, DeNardo GL, DeNardo DG, Xiong CY, Shi XB, Winthrop MD, Kroger LA, Carter P
    Clin Cancer Res 1999 Oct;5(10 Suppl):3213s-3218s
  • New anti-Cu-TETA and anti-Y-DOTA monoclonal antibodies for potential use in the pre-targeted delivery of radiopharmaceuticals to tumor
    Feng X, Pak RH, Kroger LA, Moran JK, DeNardo DG, Meares CF, DeNardo GL, DeNardo SJ
    Hybridoma 1998 Apr;17(2):125-32
  • Synergy of Taxol and radioimmunotherapy with yttrium-90-labeled chimeric L6 antibody: efficacy and toxicity in breast cancer xenografts
    DeNardo SJ, Kukis DL, Kroger LA, O'Donnell RT, Lamborn KR, Miers LA, DeNardo DG, Meares CF, DeNardo GL
    Proc Natl Acad Sci U S A 1997 Apr 15;94(8):4000-4