Grant Challen.jpg

Grant A. Challen, PhD

Assistant Professor
Department of Medicine
Oncology Division
Stem Cell Biology

Research Interests

  • Hematopoietic stem cells
  • Leukemia stem cells
  • Epigenetic modifications


  • 314-362-0987 (tel)
  • 314-362-1953 (fax)
  • Room 744 Southwest Tower (lab)
  • Washington University School of Medicine
    Division of Oncology
    Campus Box 8056
    660 South Euclid Avenue
    St. Louis, MO 63110
  • Challen Lab Website

Peer-reviewed Publications

  • PKC Epsilon Is a Key Regulator of Mitochondrial Redox Homeostasis in Acute Myeloid Leukemia
    Di Marcantonio D, Martinez E, Sidoli S, Vadaketh J, Nieborowska-Skorska M, Gupta A, Meadows JM, Ferraro F, Masselli E, Challen GA, Milsom MD, Scholl C, Froehling S, Balachandran S, Skorski T, Garcia B, Mirandola P, Gobbi G, Garzon R, Vitale M, Sykes SM
    Clin Cancer Res 2017 Nov 10; [Epub ahead of print]
  • Dnmt3a Regulates T-Cell Development and Suppresses T-ALL Transformation
    Kramer AC, Kothari A, Wilson WC, Celik H, Nikitas J, Mallaney C, Ostrander EL, Eultgen E, Martens A, Valentine MC, Young AL, Druley TE, Figueroa ME, Zhang B, Challen GA
    Leukemia 2017 Nov;31(11):2479-2490
  • The GNASR201C Mutation Associated With Clonal Hematopoiesis Supports Transplantable Hematopoietic Stem Cell Activity
    Ostrander EL, Koh WK, Mallaney C, Kramer AC, Wilson WC, Zhang B, Challen GA
    Exp Hematol 2017 Sep 19; [Epub ahead of print]
  • Clonal Haematopoiesis Harbouring AML-Associated Mutations Is Ubiquitous in Healthy Adults
    Young AL, Challen GA, Birmann BM, Druley TE
    Nat Commun 2016 Aug 22;7:12484
  • DNMT3A Loss Drives Enhancer Hypomethylation in FLT3-ITD-Associated Leukemias
    Yang L, Rodriguez B, Mayle A, Park HJ, Lin X, Luo M, Jeong M, Curry CV, Kim SB, Ruau D, Zhang X, Zhou T, Zhou M, Rebel VI, Challen GA, Gottgens B, Lee JS, Rau R, Li W, Goodell MA
    Cancer Cell 2016 Jun 13;29(6):922-934
  • Reprogrammable CRISPR/Cas9-Based System for Inducing Site-Specific DNA Methylation
    McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards JR, Challen GA
    Biol Open 2016 Jun 15;5(6):866-74
  • Enforced Differentiation of Dnmt3a-Null Bone Marrow Leads to Failure With c-Kit Mutations Driving Leukemic Transformation
    Celik H, Mallaney C, Kothari A, Ostrander EL, Eultgen E, Martens A, Miller CA, Hundal J, Klco JM, Challen GA
    Blood 2015 Jan 22;125(4):619-28
  • Dnmt3a Loss Predisposes Murine Hematopoietic Stem Cells to Malignant Transformation
    Mayle A, Yang L, Rodriguez B, Zhou T, Chang E, Curry CV, Challen GA, Li W, Wheeler D, Rebel VI, Goodell MA
    Blood 2015 Jan 22;125(4):629-38
  • Type II Interferon Promotes Differentiation of Myeloid-Biased Hematopoietic Stem Cells
    Matatall KA, Shen CC, Challen GA, King KY
    Stem Cells 2014 Nov;32(11):3023-30
  • Dnmt3a and Dnmt3b Have Overlapping and Distinct Functions in Hematopoietic Stem Cells
    Challen GA, Sun D, Mayle A, Jeong M, Luo M, Rodriguez B, Mallaney C, Celik H, Yang L, Xia Z, Cullen S, Berg J, Zheng Y, Darlington GJ, Li W, Goodell MA
    Cell Stem Cell 2014 Sep 4;15(3):350-64
  • Clonal-Level Responses of Functionally Distinct Hematopoietic Stem Cells to Trophic Factors
    Mallaney C, Kothari A, Martens A, Challen GA
    Exp Hematol 2014 Apr;42(4):317-327e2
  • Large Conserved Domains of Low DNA Methylation Maintained by Dnmt3a
    Jeong M, Sun D, Luo M, Huang Y, Challen GA, Rodriguez B, Zhang X, Chavez L, Wang H, Hannah R, Kim SB, Yang L, Ko M, Chen R, Gottgens B, Lee JS, Gunaratne P, Godley LA, Darlington GJ, Rao A, Li W, Goodell MA
    Nat Genet 2014 Jan;46(1):17-23
  • Dnmt3a Is Essential for Hematopoietic Stem Cell Differentiation
    Challen GA, Sun D, Jeong M, Luo M, Jelinek J, Berg JS, Bock C, Vasanthakumar A, Gu H, Xi Y, Liang S, Lu Y, Darlington GJ, Meissner A, Issa JP, Godley LA, Li W, Goodell MA
    Nat Genet 2011 Dec 4;44(1):23-31
  • Investigating the Role of Hematopoietic Stem and Progenitor Cells in Regulating the Osteogenic Differentiation of Mesenchymal Stem Cells in Vitro
    Liao J, Hammerick KE, Challen GA, Goodell MA, Kasper FK, Mikos AG
    J Orthop Res 2011 Oct;29(10):1544-53
  • Distinct Hematopoietic Stem Cell Subtypes Are Differentially Regulated by TGF-Beta1
    Challen GA, Boles NC, Chambers SM, Goodell MA
    Cell Stem Cell 2010 Mar 5;6(3):265-78
  • Runx1 Isoforms Show Differential Expression Patterns During Hematopoietic Development but Have Similar Functional Effects in Adult Hematopoietic Stem Cells
    Challen GA, Goodell MA
    Exp Hematol 2010 May;38(5):403-16
  • Promiscuous Expression of H2B-GFP Transgene in Hematopoietic Stem Cells
    Challen GA, Goodell MA
    PLoS One 2008 Jun 4;3(6):e2357
  • Kidney Side Population Reveals Multilineage Potential and Renal Functional Capacity but Also Cellular Heterogeneity
    Challen GA, Bertoncello I, Deane JA, Ricardo SD, Little MH
    J Am Soc Nephrol 2006 Jul;17(7):1896-912
  • Definition and Spatial Annotation of the Dynamic Secretome During Early Kidney Development
    Martinez G, Georgas K, Challen GA, Rumballe B, Davis MJ, Taylor D, Teasdale RD, Grimmond SM, Little MH
    Dev Dyn 2006 Jun;235(6):1709-19
  • Temporal and Spatial Transcriptional Programs in Murine Kidney Development
    Challen G, Gardiner B, Caruana G, Kostoulias X, Martinez G, Crowe M, Taylor DF, Bertram J, Little M, Grimmond SM
    Physiol Genomics 2005 Oct 17;23(2):159-71
  • Identifying the Molecular Phenotype of Renal Progenitor Cells
    Challen GA, Martinez G, Davis MJ, Taylor DF, Crowe M, Teasdale RD, Grimmond SM, Little MH
    J Am Soc Nephrol 2004 Sep;15(9):2344-57